Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Decreased CD44v3 expression impairs endometrial stromal cell proliferation and decidualization in women with recurrent implantation failure

doi: 10.1186/s12958-022-01042-w

Figure Lengend Snippet: Demographic characteristics of women recruited in this study

Article Snippet: Human telomerase reverse transcriptase-immortalized human endometrial stromal cells (T-HESCs; which are commonly used to study HESCs and endometrial receptivity) and Ishikawa cells (human endometrial carcinoma cell line, which are commonly used to study endometrial epithelial cells and embryo adhesion), were acquired from the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK).

Techniques:

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Decreased CD44v3 expression impairs endometrial stromal cell proliferation and decidualization in women with recurrent implantation failure

doi: 10.1186/s12958-022-01042-w

Figure Lengend Snippet: Expression of CD44v3 in the endometrium of women with RIF ( A ) mRNA levels of CD44s, CD44v3, and CD44v6 in the endometrium of women in the control ( n = 18) and RIF ( n = 24) groups. B Immunohistochemical staining and ( C ) semiquantitative analysis of CD44s, CD44v3, and CD44v6 in the control and RIF groups ( n = 15 per group). D Representative western blots and ( E ) densitometric quantification of CD44v3 in the control and RIF groups. (F) ROC curve for determining the expression of CD44v3 in endometrial tissue. Bar = 50 µm. * p < 0.05; ** p < 0.01

Article Snippet: Human telomerase reverse transcriptase-immortalized human endometrial stromal cells (T-HESCs; which are commonly used to study HESCs and endometrial receptivity) and Ishikawa cells (human endometrial carcinoma cell line, which are commonly used to study endometrial epithelial cells and embryo adhesion), were acquired from the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK).

Techniques: Expressing, Control, Immunohistochemical staining, Staining, Western Blot

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Decreased CD44v3 expression impairs endometrial stromal cell proliferation and decidualization in women with recurrent implantation failure

doi: 10.1186/s12958-022-01042-w

Figure Lengend Snippet: Expression of CD44v3 in endometrial tissues and primary endometrial cells after E 2 and P 4 treatments (A)Immunohistochemical staining and (B) semiquantitative analysis of CD44v3 during the proliferative ( n = 12) and mid-secretory phases ( n = 15) in the control patients. (C) Western blot and (D) statistical analyses of CD44v3 expression in the endometrium during the proliferative and mid-secretory phases in the control group. (E and G) Western blot and (F and H) statistical analyses of CD44v3 expression in primary HESCs and HEECs with or without estrogen (E 2 ) and progesterone (P 4 ) treatment for 72 h. P, proliferative phase; MS, mid-secretory phase. Bar = 50 µm. * p < 0.05.

Article Snippet: Human telomerase reverse transcriptase-immortalized human endometrial stromal cells (T-HESCs; which are commonly used to study HESCs and endometrial receptivity) and Ishikawa cells (human endometrial carcinoma cell line, which are commonly used to study endometrial epithelial cells and embryo adhesion), were acquired from the European Collection of Authenticated Cell Cultures (ECACC; Salisbury, UK).

Techniques: Expressing, Immunohistochemical staining, Staining, Control, Western Blot

Journal: Frontiers in Immunology

Article Title: Staphylococcus epidermidis prevents UV-induced skin aging by suppressing TLR3-mediated senescence

doi: 10.3389/fimmu.2026.1796085

Figure Lengend Snippet: Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human fibroblasts for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Neonatal human epidermal keratinocytes (NHEKs) and neonatal human fibroblasts were purchased from Lifeline Cell Technology.

Techniques: Irradiation, Cell Culture, Centrifugation, Concentration Assay, Staining, Western Blot, Reverse Transcription Polymerase Chain Reaction, Isolation, Expressing, Control